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primary antibodies against col2a1  (Santa Cruz Biotechnology)
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EGCG Attenuates IL-1β-Induced Inflammatory Response and Catabolism in Chondrocytes. Chondrocytes were treated with IL-1β (10 ng/mL) in the presence or absence of EGCG (1, 10, 50 μM) for 24 hours. The expression levels of inflammation- and catabolism-related proteins in chondrocytes were subsequently evaluated. ( a-e ) Western blot analysis of iNOS, MMP13, COX2, and IL-6 expression. ( f-i ) Immunofluorescence co-staining with DAPI to detect <t>COL2A1</t> and MMP13 in the nucleus (scale bar: 100 μm). The data in the figures are expressed as means ± S.D. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns p > 0.05, n = 3.
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EGCG Attenuates IL-1β-Induced Inflammatory Response and Catabolism in Chondrocytes. Chondrocytes were treated with IL-1β (10 ng/mL) in the presence or absence of EGCG (1, 10, 50 μM) for 24 hours. The expression levels of inflammation- and catabolism-related proteins in chondrocytes were subsequently evaluated. ( a-e ) Western blot analysis of iNOS, MMP13, COX2, and IL-6 expression. ( f-i ) Immunofluorescence co-staining with DAPI to detect <t>COL2A1</t> and MMP13 in the nucleus (scale bar: 100 μm). The data in the figures are expressed as means ± S.D. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns p > 0.05, n = 3.
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EGCG Attenuates IL-1β-Induced Inflammatory Response and Catabolism in Chondrocytes. Chondrocytes were treated with IL-1β (10 ng/mL) in the presence or absence of EGCG (1, 10, 50 μM) for 24 hours. The expression levels of inflammation- and catabolism-related proteins in chondrocytes were subsequently evaluated. ( a-e ) Western blot analysis of iNOS, MMP13, COX2, and IL-6 expression. ( f-i ) Immunofluorescence co-staining with DAPI to detect <t>COL2A1</t> and MMP13 in the nucleus (scale bar: 100 μm). The data in the figures are expressed as means ± S.D. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns p > 0.05, n = 3.
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EGCG Attenuates IL-1β-Induced Inflammatory Response and Catabolism in Chondrocytes. Chondrocytes were treated with IL-1β (10 ng/mL) in the presence or absence of EGCG (1, 10, 50 μM) for 24 hours. The expression levels of inflammation- and catabolism-related proteins in chondrocytes were subsequently evaluated. ( a-e ) Western blot analysis of iNOS, MMP13, COX2, and IL-6 expression. ( f-i ) Immunofluorescence co-staining with DAPI to detect <t>COL2A1</t> and MMP13 in the nucleus (scale bar: 100 μm). The data in the figures are expressed as means ± S.D. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns p > 0.05, n = 3.
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EGCG Attenuates IL-1β-Induced Inflammatory Response and Catabolism in Chondrocytes. Chondrocytes were treated with IL-1β (10 ng/mL) in the presence or absence of EGCG (1, 10, 50 μM) for 24 hours. The expression levels of inflammation- and catabolism-related proteins in chondrocytes were subsequently evaluated. ( a-e ) Western blot analysis of iNOS, MMP13, COX2, and IL-6 expression. ( f-i ) Immunofluorescence co-staining with DAPI to detect COL2A1 and MMP13 in the nucleus (scale bar: 100 μm). The data in the figures are expressed as means ± S.D. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns p > 0.05, n = 3.

Journal: Journal of Inflammation Research

Article Title: Integration of Network Pharmacology, Molecular Docking, and Experimental Validation to Identify the Effect of EGCG on Alleviating Chondrocyte Inflammatory Damage by Targeting ER Stress-STAT3 Crosstalk

doi: 10.2147/JIR.S564356

Figure Lengend Snippet: EGCG Attenuates IL-1β-Induced Inflammatory Response and Catabolism in Chondrocytes. Chondrocytes were treated with IL-1β (10 ng/mL) in the presence or absence of EGCG (1, 10, 50 μM) for 24 hours. The expression levels of inflammation- and catabolism-related proteins in chondrocytes were subsequently evaluated. ( a-e ) Western blot analysis of iNOS, MMP13, COX2, and IL-6 expression. ( f-i ) Immunofluorescence co-staining with DAPI to detect COL2A1 and MMP13 in the nucleus (scale bar: 100 μm). The data in the figures are expressed as means ± S.D. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns p > 0.05, n = 3.

Article Snippet: Primary antibodies against COL2A1 (sc-28887), MMP13 (sc-30073), Matrix Metallopeptidase 3 (MMP3) (sc-21732), and SRY-box transcription factor 9(SOX9) (sc-20095) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Expressing, Western Blot, Immunofluorescence, Staining

EGCG reduces chondrocyte apoptosis; TM-induced ER stress reversed the protective effects of EGCG against IL-1β-induced matrix reduction in chondrocytes. ( a and b ) COL2A1 immunofluorescence staining. Markedly increased green bright puncta indicated the upregulated expression of COL2A1 (bar: 100 μm). ( c and d ) SOX9 immunofluorescence staining. Markedly increased red bright puncta indicated the upregulated expression of SOX9 (bar: 100 μm). Fluorescence intensity was quantified using ImageJ software. The data in the figures are expressed as means ± S.D., **p < 0.01, ***p < 0.001, ns p > 0.05; n = 3.

Journal: Journal of Inflammation Research

Article Title: Integration of Network Pharmacology, Molecular Docking, and Experimental Validation to Identify the Effect of EGCG on Alleviating Chondrocyte Inflammatory Damage by Targeting ER Stress-STAT3 Crosstalk

doi: 10.2147/JIR.S564356

Figure Lengend Snippet: EGCG reduces chondrocyte apoptosis; TM-induced ER stress reversed the protective effects of EGCG against IL-1β-induced matrix reduction in chondrocytes. ( a and b ) COL2A1 immunofluorescence staining. Markedly increased green bright puncta indicated the upregulated expression of COL2A1 (bar: 100 μm). ( c and d ) SOX9 immunofluorescence staining. Markedly increased red bright puncta indicated the upregulated expression of SOX9 (bar: 100 μm). Fluorescence intensity was quantified using ImageJ software. The data in the figures are expressed as means ± S.D., **p < 0.01, ***p < 0.001, ns p > 0.05; n = 3.

Article Snippet: Primary antibodies against COL2A1 (sc-28887), MMP13 (sc-30073), Matrix Metallopeptidase 3 (MMP3) (sc-21732), and SRY-box transcription factor 9(SOX9) (sc-20095) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Immunofluorescence, Staining, Expressing, Fluorescence, Software